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why does nobody talk about test results

Discussion Clean Column ×4 Slow Clap ×3

Asking properly rather than in a comment on somebody else’s thread: why does nobody talk about test results.

Tested three lots over a year rather than three vials from one lot. Cost the same and told me something completely different.

Sent a vial in specifically to check a supplier’s claim after a good experience. It matched. Boring result, worth logging.

Have now spent more on testing than on some of the orders. No regrets — the log is the reason I can buy without agonising.

Happy to answer the boring questions. Those are usually the ones worth asking.

2,895 up / 667 down81% upvoted38 commentsid 1rkj1s22 Dec 2023

38 comments

30 in this archive, depth 5

best — the order this archive was captured in

u/camila_mensa193 points·2 years ago

Area percent and net peptide content are different measurements and most reports give one. Quote which, or the comparison downstream is meaningless.

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u/ffmi_watcher162 points·2 years ago

Area percent and net peptide content are different measurements and most reports give one.

Adding the boring but useful bit: say whether you paid for it. It is the basis of the whole log.

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u/camila_mensa227 points·2 years ago

the log is cumulative, that is the whole value of it

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u/saskia_mwangi44 points·2 years ago

Area percent and net peptide content are different measurements and most reports give one.

This is why the template has a batch field. Without it the result is about a vial nobody can identify.

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u/georgi_chowdhury16 points·2 years ago

This is why the template has a batch field.

saskia_mwangi is right that the band matters more than the peak. Consistency across lots is the finding.

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[removed]29 points·2 years ago

[removed by moderator]

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u/peptidemeter_pat14 points·2 years ago

if you are going to test, test the thing you actually received

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u/usp_appendixcompendial7 points·2 years ago

one result is a data point, agreement between two services is evidence

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u/runa_wikstrom121 points·2 years ago

Inter-lab variance on this assay runs roughly a point either way, driven mostly by gradient and integration. Two results inside that band agree; they do not disagree quietly.

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u/saskia_mwangi35 points·2 years ago

Not convinced by the comparison. Different services, different gradients, and you are treating one as ground truth.

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u/stubborn_batchlist_onlyOP14 points·2 years ago

Batch number and date of analysis?

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u/stubborn_batchlist_onlyOP27 points·2 years ago

What was the claim on the certificate you are comparing against?

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u/stubborn_batchlist_onlyOP19 points·2 years ago

Area percent or net peptide content?

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u/neha_rahimi8 points·2 years ago

date of analysis matters as much as the number

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u/nayeli_iyer89 points·2 years ago

CPC BPC, batch matched the paperwork, Janoshik came back 99.3% against a claimed 98.5%. Paid for it myself, posting it because the log is only worth anything if people do.

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u/tomas_lundgren42 points·2 years ago·edited

Same. The KP results have been sitting in a tight band across lots, which is the useful shape.

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u/cold_chromatogram_only10 points·2 years ago

vendor, compound, batch, service, result — that is the template

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u/crp_curious39 points·2 years ago

Split one vial between VendorInvestigate and PeptideMeter. The two numbers were within half a point of each other and I stopped worrying about which lab to trust.

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u/whois_wanda31 points·2 years ago

If the report gives a mass spectrum as well as UV purity, say so. Identity and relative quantity are separate claims and the log tracks both.

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u/endotoxin_elliemicro13 points·2 years ago

Is that the same lot as the one in the thread from last month?

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u/aksel_jansen9 points·2 years ago

Kept every report as a PDF with the batch in the filename. Two years in, it is the most useful folder I own.

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u/emil_marchand-31 points·2 years ago

net peptide content if the report gives it, and say which you are quoting

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u/saskia_mwangi-37 points·2 years ago

Chain of custody is why member-funded testing is worth more than a supplied certificate: the vial tested is the vial that arrived at a member’s door.

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u/ffmi_watcher1 point·2 years ago

Did the vial lot match the paperwork lot?

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u/yannick_barros1 point·2 years ago

That service reports area percent by default. Worth stating, since the thread has been comparing it to a mass figure.

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u/arne_restrepo1 point·2 years ago

the trend across lots is worth more than any single figure

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u/hugo_pires15 points·2 years ago

The template is vendor, compound, batch, service, claim, result, and who paid. Every one of those fields exists because a result missing it has been argued about here before.

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u/asks_dumb_questions12 points·2 years ago

A series across separate lots estimates a process. Repeat tests on one lot estimate the measurement. Both are useful and they answer different questions.

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u/whois_wanda10 points·2 years ago

Disagree that this is a discrepancy. 99.6% against a claimed 99.0% is inside ordinary inter-lab variance in either direction.

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u/endotoxin_elliemicro7 points·2 years ago

Agreed on quoting the claim alongside the result. Half the arguments here are two people comparing to different reference numbers.

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A running record of independent test results people paid for themselves. Every post follows the same shape: vendor, product, batch, testing service, method, and the number. Good results and bad results are equally welcome and get equal scrutiny.

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