GLPHubglpresearchhub.com
Read-only archive. GLP Research Hub is a static community record — nothing here is for sale, no account is needed, and no vote you cast is counted. Why?
ST

u/step_count_stan

8,000 steps is not exercise, it is a floor. Build from there.

member · joined 6 Apr 2024

6,285post karma
5,244comment karma
5submissions
85comments

Comments — page 3 of 3

comment on [Question] is 94.2% actually fine or am I being sold a rounding error in c/hplc · 51 points · 2 years ago

Correction: that is a tailing factor, not a plate count. Different diagnostics for different problems.

permalink

comment on chromatogram is the most under-discussed thing on this board in c/hplc · 32 points · 2 years ago

Yes. Retention time is a hypothesis about identity. Mass is the answer.

permalink

comment on [Discussion] we are measuring testing at the wrong time and calling it noise in c/vendorvetting · 1 points · 2 years ago

Minimum order and lead time — did they quote both up front?

permalink

comment on how much of what we believe about HPLC actually comes from gradient threads in c/hplc · 123 points · 2 years ago

Push back: a longer run is not automatically better resolution. You are trading peak width for time and the ratio is what matters.

permalink

comment on purity — 19 things I got wrong before I got it right in c/hplc · 657 points · 2 years ago

A shoulder that does not baseline-resolve cannot be quantified honestly. You can report it as an unresolved shoulder, which is useful information, or you can develop the method until it resolves.

permalink

Trophy case
Top Contributor Answered the Question
Cake day 6 Apr 2024
Sponsored

Sigma-Aldrich Standards

Certified reference materials for peptide identity and purity work.

sigmaaldrich.com
Where they post
Before you read on

Several compounds discussed on GLP Research Hub are sold for research use only and are not approved for human use anywhere. Nothing here is medical advice and none of it is written by your clinician. If a post reads like an instruction, treat it as a description of what one stranger did.