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c/reconstitution·posted 2 years ago by u/cesar_ivaturi

[Question] how do you actually verify mg/mL

Question Clean Column ×3 Long Haul ×2

how do you actually verify mg/mL. I would rather ask a basic question now than get this wrong quietly for two months.

Adding more water does not change how much peptide is in the vial. It changes the concentration, which changes the volume you draw for the same amount. That is the entire relationship.

Bacteriostatic water contains benzyl alcohol as a preservative, which is what makes repeated entry into the same vial reasonable. Plain sterile water has no preservative.

The lyophilised cake displaces a small volume, so the true concentration is very slightly lower than the arithmetic suggests. It is a fraction of a percent and it is dwarfed by fill tolerance.

I will update this if the picture changes rather than quietly leaving it up.

2,547 up / 802 down76% upvoted32 commentsid k1ax8y27 Jun 2024

32 comments

13 in this archive, depth 4

best — the order this archive was captured in

u/dilara_szabo0 points·2 years ago

The arithmetic in full: concentration is mass over volume, so 10mg into 1mL gives 10mg/mL. A U-100 syringe is graduated at 100 units per millilitre, so 2mg is 2 divided by 10 millilitres, which is 20 units.

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u/vial_math_veraMOD175 points·2 years ago

Left up and flaired Explainer — the numbers in this one check out.

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u/cesar_ivaturiOP98 points·2 years ago

Are you accounting for the powder volume, or is that where the confusion is?

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u/runa_cabrera23 points·2 years ago

label the vial with the date as well as the concentration

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u/gradient_goblin5 points·2 years ago

Wrote the date on the vial as well as the concentration after losing track of which one was opened first.

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u/hedda_ekstrom154 points·2 years ago

more water is not more drug, it is the same drug in a bigger volume

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u/marta_weiss52 points·2 years ago

bacteriostatic water has the preservative, sterile water does not

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u/units_not_mgsyringe math43 points·2 years ago

Reconstituted at 1mL when I meant to use a different volume. Caught it because the units looked wrong on the barrel. Now I write 5mg/mL on the glass before I put the cap back.

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u/cesar_ivaturiOP34 points·2 years ago

Correcting myself above: I wrote the concentration the wrong way up. Mass over volume, not volume over mass.

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u/cesar_ivaturiOP17 points·2 years ago

What is written on the vial right now?

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u/rosa_mensah46 points·2 years ago

aim the stream at the glass wall, not at the cake

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u/RetaAndRegret289 points·2 years ago

Bacteriostatic or plain sterile water?

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u/elin_ferrari50 points·2 years ago

Correction: that gives 30 units, not what you have written. 15mg into 3mL is 5mg/mL and the division follows from there.

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The arithmetic and the technique. Bacteriostatic versus sterile water, reconstitution volume as a concentration choice, converting mg/mL into insulin-syringe units, dead space, swirl-not-shake, stopper coring, and how long a reconstituted vial is actually good for. Math errors get corrected fast and politely.

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