GLPHubglpresearchhub.com
Read-only archive. GLP Research Hub is a static community record — nothing here is for sale, no account is needed, and no vote you cast is counted. Why?
Archived. This submission is more than a year old. Votes and new comments are closed, and some of the advice in it may have been superseded — check the community wiki for the current version.
8.8k
c/reconstitution·posted 2 years ago by u/janek_ferrari

[Meta] proposal — a flair for units posts

PSA Cold Box ×4 Slow Clap ×3 Clean Column ×3

proposal — a flair for units posts, and it will stay as it is unless somebody makes the case to change it.

I keep a laminated card in the drawer with the arithmetic on it. Looks ridiculous. Has never once let me draw the wrong volume.

Research-use-only material is not approved for human use, so treat everything in this thread as arithmetic about solutions rather than instructions for a person.

Reconstituted at 1mL when I meant to use a different volume. Caught it because the units looked wrong on the barrel. Now I write 20mg/mL on the glass before I put the cap back.

Ask me anything specific. Anything general I will probably get wrong.

10,573 up / 1,801 down85% upvoted59 commentsid erlmkg16 May 2024

59 comments

22 in this archive, depth 5

best — the order this archive was captured in

u/viktor_erdogan1.3k points·2 years ago

Adding more water does not change how much peptide is in the vial. It changes the concentration, which changes the volume you draw for the same amount. That is the entire relationship.

replysharereportpermalink
u/india_generic_g834 points·2 years ago

Cosigning the round-number approach. Reconstituting to something awkward is how you end up drawing 27.5 units at six in the morning.

replysharereportpermalink
u/quarter_dose_queenlow and slow498 points·2 years ago

Cosigning the round-number approach.

Adding one thing — put the date on the label too. Concentration plus date and you can never mix two vials up.

replysharereportpermalink
u/janek_ferrariOP302 points·2 years ago

How many mg in the vial and how many mL are you adding?

replysharereportpermalink
u/viktor_kirchner-19 points·2 years ago

more water is not more drug, it is the same drug in a bigger volume

replysharereportpermalink
u/ilias_cabrera1 point·2 years ago

Wrote the date on the vial as well as the concentration after losing track of which one was opened first.

replysharereportpermalink
u/priya_guerrero246 points·2 years ago

the powder cake sits at the bottom, the volume you add is the volume that counts

replysharereportpermalink
u/quiet_reader_99741 points·2 years ago

Aim the stream down the wall of the vial rather than into the cake. Reconstitution is a solubility problem, not a mixing problem, and mechanical shear does nothing helpful.

replysharereportpermalink
u/lurker_for_years265 points·2 years ago

swirl, never shake

replysharereportpermalink
u/runa_grimaldi568 points·2 years ago

The dead space question sent me down a rabbit hole for a week. It is a fraction of a unit and it is the same every draw.

replysharereportpermalink
[removed]373 points·2 years ago

[removed by moderator]

replysharereportpermalink
u/ines_ekstrom242 points·2 years ago

That is sterile water, not bacteriostatic. Same appearance, different product, and it matters for a multi-draw vial.

replysharereportpermalink
load more comments (1) →
u/sock_puppet_spotter521 points·2 years ago

Asked GGPeps what volume they reconstitute to for their own stability testing and they answered in one line. Useful thing to know.

replysharereportpermalink
u/bac_water_banditMOD344 points·2 years ago

Working has to be shown on arithmetic posts. Added a note asking for it rather than removing this one.

replysharereportpermalink
u/emil_zamora507 points·2 years ago

Bacteriostatic water contains benzyl alcohol as a preservative, which is what makes repeated entry into the same vial reasonable. Plain sterile water has no preservative.

replysharereportpermalink
u/anjali_tamm198 points·2 years ago

a U-100 syringe reads 100 units to the millilitre, every time

replysharereportpermalink
u/batch_number_bertievetting211 points·2 years ago

Working has to be shown on arithmetic posts.

Right, and the arithmetic behind it: 30mg in 3mL is 10mg/mL, so 2.5mg is 25 units.

replysharereportpermalink
u/rina_bergstrom136 points·2 years ago

Disagree with the "more water is safer" framing. It is not safer, it is just a different number to divide by.

replysharereportpermalink
u/swirl_dont_shakereconstitution158 points·2 years ago

Spent 13 weeks confused about this until somebody drew the table for me. 15mg, 3mL, 5mg/mL, 0.5mg is 10 units. That is the whole thing.

replysharereportpermalink
u/janek_ferrariOP254 points·2 years ago

Working has to be shown on arithmetic posts.

bac_water_bandit has the units point exactly right. They are a volume on the barrel and nothing else.

replysharereportpermalink
u/adaeze_cabrera254 points·2 years ago

I would not round that figure. At 5mg/mL a rounding error of two units is a real fraction of the intended amount.

replysharereportpermalink
Permalinked branches
Deep branches get their own page so a single reply chain can be linked and read on its own.
About c/reconstitution

The arithmetic and the technique. Bacteriostatic versus sterile water, reconstitution volume as a concentration choice, converting mg/mL into insulin-syringe units, dead space, swirl-not-shake, stopper coring, and how long a reconstituted vial is actually good for. Math errors get corrected fast and politely.

88kmembers
130submissions
Aug 2023created
submissions / month, last year
Sponsored

Sigma-Aldrich Standards

Certified reference materials for peptide identity and purity work.

sigmaaldrich.com
c/reconstitution rules
  1. Show your numbers. "How many units?" with no mg and no mL cannot be answered.
  2. Never post a calculation as advice without stating the concentration it assumes.
  3. No sterile-compounding-for-others discussion. Personal handling only.
  4. Independent community. Nobody here sells anything, and anyone who tries is banned.
  5. Not medical advice. Describe what you did; never prescribe to a stranger.
  6. Claims need evidence. Batch numbers, dated screenshots, independent test reports, or a citation.
  7. No referral links, discount codes or affiliate URLs. Permanent ban, no appeal.
  8. No contact handles, wallet addresses or tracking numbers — they identify people.
  9. Be recognisably decent. Disagree hard, insult nobody.
Moderators
Volunteers. Unpaid, unaffiliated, and reachable through modmail only.
Before you read on

Several compounds discussed on GLP Research Hub are sold for research use only and are not approved for human use anywhere. Nothing here is medical advice and none of it is written by your clinician. If a post reads like an instruction, treat it as a description of what one stranger did.