integration — 22 things I got wrong before I got it right
integration — 22 things I got wrong before I got it right. It is the sort of thing everyone half-believes and nobody writes down. Resolution between two peaks depends on retention, selectivity and efficiency. A shallower gradient buys retention and usually resolution, at the cost of peak width and run time. UV…
Left up. The integration disagreement in this thread is the most useful thing on the board this week.
Area percent is the integrated area of your peak over the total integrated area at one wavelength on one gradient. Change any of those and the number changes without the sample changing.
Area percent is the integrated area of your peak over the total integrated area at one wavelength on one gradient.
Disagreeing with this bit: that spread is ordinary inter-lab variance, not a disagreement about the material.
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That figure cannot be quoted to two decimals off that baseline. The precision is not in the data.
Agreed, and it is why GL Biochem printing the column and gradient on the certificate is genuinely useful rather than decorative.
ghost peak, check the plumbing first, it is always the plumbing
214nm sees the peptide bond, 280nm sees the aromatics
reproducibility beats resolution if you only get one of them
Small fix — 214nm, not 210. It matters for the comparison you are making with the other run.
integration decisions move the number more than the sample does
Yes. Retention time is a hypothesis about identity. Mass is the answer.