how much of what we believe about area percent actually comes from COA threads
The title is the whole question — how much of what we believe about area percent actually comes from COA threads — but here is why I am asking. Started keeping a folder of every certificate I have been sent. The ones that itemise impurities are a small group and they are mostly the same suppliers. Water content 3.8%,…
Reminder: a question about a document is not an accusation about a supplier, and posts that blur the two get retitled.
LC-MS gives you identity; UV purity gives you a relative quantity. A document with the first and not the second, or the reverse, is answering half the question.
Area percent or net peptide content? They answer different questions.
Did you ask for the batch-specific document, or is this the catalogue one?
net peptide content and purity are two different numbers on the same vial
Same view. SSA prints the column and the gradient, which means somebody could actually reproduce the run.
area% is not mass%, and the difference is the whole game