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c/coa·submitted 1 year ago by u/clara_danquah

unpopular opinion: most of what gets said here about certificate of analysis is guesswork

Labbranch of 9 comments

unpopular opinion: most of what gets said here about certificate of analysis is guesswork — a position I have arrived at slowly and would like tested. LC-MS gives you identity; UV purity gives you a relative quantity. A document with the first and not the second, or the reverse, is answering half the question. Asked…

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9 comments, started 1 year ago
u/purity_pedantMOD265 points·1 year ago

Your name and address edited out of the screenshot above. The batch details are untouched and should stay.

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u/ignacio_silva0 points·1 year ago

Your name and address edited out of the screenshot above.

Agreed, and the practical version is: ask for the itemised table. If it exists, you get it.

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u/anya_moreau106 points·1 year ago

Pushing back a little: a missing field is usually presentation, not concealment. Ask for the full document before drawing conclusions.

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u/hassan_chowdhury51 points·1 year ago

Agreed on asking for the storage condition. It is one line and it changes how you read the entire stability claim.

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u/yusuf_nakamura76 points·1 year ago

Area percent or net peptide content? They answer different questions.

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u/tove_ogunleye177 points·1 year ago·edited

Did you ask for the batch-specific document, or is this the catalogue one?

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u/aleksi_eriksen133 points·1 year ago

Not quite. Two labs with different gradients can resolve a close-eluting impurity differently and both be reporting honestly.

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u/nadia_kjaer87 points·1 year ago

Disagree that a lower number is automatically worse. A conservative claim that survives testing is worth more than an optimistic one that does not.

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About c/coa

How to read a certificate of analysis and what it does and does not tell you. Area% versus mass%, identity versus quantity, water content, related substances, acetate counterions, and the difference between a vendor-supplied COA and an independent test on the vial you actually received.

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