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c/bpc157·posted 2 years ago by u/anya_erdogan

someone explain pentadecapeptide to me like I have not read a paper in years

Question Long Haul ×4

Question in the title, detail here: someone explain pentadecapeptide to me like I have not read a paper in years.

It is a fifteen-residue peptide with a published sequence, which makes synthesis straightforward and makes identity verification the meaningful test rather than an optional extra.

Short peptides in solution are subject to hydrolysis and, depending on sequence, oxidation. Storage state and time in solution are practical variables that this board almost never discusses.

The published literature is overwhelmingly preclinical, largely in rodent models. Translating a rodent result into a human expectation is not a small step, and almost every confident claim here takes it silently.

Happy to answer the boring questions. Those are usually the ones worth asking.

526 up / 200 down72% upvoted23 commentsid ztal27 Apr 2024

23 comments

20 in this archive, depth 4

best — the order this archive was captured in

u/nadia_nascimento33 points·2 years ago

Purity by area percent tells you the sample is homogeneous. Mass spectrometry tells you what the homogeneous thing is. For a short peptide, the second question is the one worth paying for.

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u/rekha_mwangi27 points·2 years ago

preclinical enthusiasm is not clinical evidence

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u/enzo_fonseca26 points·2 years ago

Which paper is that claim from, and was it in humans?

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u/ismael_chukwu16 points·2 years ago·edited

Not convinced. A high purity figure with no identity confirmation tells you the sample is homogeneous, not that it is what you ordered.

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u/anya_erdoganOP12 points·2 years ago·edited

pentadecapeptide, fifteen residues, and the sequence is public

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u/milos_mensa8 points·2 years ago

ask for the mass spectrum, not just the purity number

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u/anya_erdoganOP12 points·2 years ago

read the actual papers, they are more modest than the summaries

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u/search_before_post0 points·2 years ago

read the actual papers, they are more modest than the summaries

This is the point that should be at the top of every thread here. Identity first.

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u/throwaway_91821 point·2 years ago

This is the point that should be at the top of every thread here.

Agreed — and the preclinical to clinical gap is where nearly all the overclaiming happens.

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u/joaquin_ivaturi4 points·2 years ago

Regulatory classification differs between jurisdictions and has changed in several of them recently. Any answer to a status question needs a place and a date attached.

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u/throwaway_91821 point·2 years ago

Yes — the rodent literature is real and it is rodent literature. The gap to a human claim is enormous and it gets crossed in one sentence here.

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u/teodor_szabo8 points·2 years ago

Push back: "no reported harms" in a literature with almost no human trials is not a safety finding.

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u/marit_sandvik5 points·2 years ago

Cosigning that the papers are more modest than the summaries of them. That is true across this whole site and especially here.

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u/enzo_fonseca0 points·2 years ago

Ask for the theoretical mass alongside the measured one. It is a one-line addition to a certificate and it converts a purity claim into an identity claim.

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u/ruben_cabrera1 point·2 years ago

sequence confirmation is the test worth paying for

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u/anya_erdoganOP3 points·2 years ago

Sent a vial to PeptideMeter: 99.6% against a claimed 99.0%, with the mass confirming the sequence. Both halves mattered to me.

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u/zaid_eriksen2 points·2 years ago

a fifteen-residue peptide is easy to make and easy to make badly

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u/ms_fragmenter0 points·2 years ago

a fifteen-residue peptide is easy to make and easy to make badly

zaid_eriksen is right that a pure something-else is still something else.

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u/signe_villalobos1 point·2 years ago

identity, quantity, stability — three separate questions

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u/bruno_dumitru2 points·2 years ago

short peptides are cheap, which cuts both ways

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About c/bpc157

BPC-157 as it actually is: a pentadecapeptide with a genuinely interesting rodent tendon-and-gut literature, near-zero controlled human data, a naming and sequence mess in the supply chain, and a regulatory status that changed sharply in several countries.

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