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[Lab] LC-MS confirmed identity but the UV purity is 94%. now what
Research peptide, not a GLP-1, from a vendor I will not name because this is a methodology question rather than a vendor post. MS confirms the expected molecular mass…
u/mass_spec_maggie
LC-MS/MS is my day job. Peptide mapping is my hobby. Both are the same job.
long-standing contributor · MS · joined 19 Jan 2024
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3k
constipation: the version I wish someone had shown me in week 1
constipation: the version I wish someone had shown me in week 1. Same spreadsheet I have been keeping since the start, nothing retrofitted. A symptom that begins the…
1.1k
[Discussion] can we stop arguing about dose stretching until somebody posts a number
Question in the title, detail here: can we stop arguing about dose stretching until somebody posts a number. Drifted up 6kg over a winter and panicked. It came back off…
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how much of what we believe about HPLC actually comes from integration threads
Genuine question, and the title is the question: how much of what we believe about HPLC actually comes from integration threads. Started reporting my own integrations…
7k
why does nobody talk about gradient
Genuine question, and the title is the question: why does nobody talk about gradient. UV response is not uniform across species. At 214nm you are looking at the peptide…