[Lab] 16th independent test on ERP — 97.4% on a claimed 97.0%, and the trend is the interesting part
Numbers in the title, numbers in the post. 16th independent test on ERP — 97.4% on a claimed 97.0%, and the trend is the interesting part. Since the title puts numbers in the shop window: 97.4% and 97.0%. Gave the same trace to two people I trust and got two integrations about a point apart. That was the most…
Area percent is the integrated area of your peak over the total integrated area at one wavelength on one gradient. Change any of those and the number changes without the sample changing.
214nm sees the peptide bond, 280nm sees the aromatics
Correction: that is a tailing factor, not a plate count. Different diagnostics for different problems.
What wavelength, and what was the gradient?
What did system suitability look like on that sequence?
Area percent is the integrated area of your peak over the total integrated area at one wavelength on one gradient.
This is the whole methodological point of the board in one line.
This is the whole methodological point of the board in one line.
Agreed. And the corollary is that the method line on a certificate is not decoration, it is the number’s provenance.
This. A shoulder that does not baseline-resolve is a question, not a quantity.
Small fix — 214nm, not 210. It matters for the comparison you are making with the other run.