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c/hplc·submitted 9 months ago by u/arne_nyberg

how much of what we believe about integration actually comes from LC-MS threads

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how much of what we believe about integration actually comes from LC-MS threads. Searched first, found three threads that contradict each other, hence the post. Chased a ghost peak for three weeks. It was the plumbing. It is always the plumbing. A shoulder that does not baseline-resolve cannot be quantified honestly.…

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6 comments, started 9 months ago
u/vikram_asante263 points·9 months ago

axis labels or the trace is decoration

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u/priya_ogunleye111 points·9 months ago

Yes — system suitability first. Without it the number is an assertion about the instrument, not the sample.

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u/mass_spec_maggieMS103 points·9 months ago

Agreed, and it is why GL Biochem printing the column and gradient on the certificate is genuinely useful rather than decorative.

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u/ahmed_rasmussen60 points·9 months ago

Can you post the trace with the axes labelled?

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u/baseline_drifteranalytical56 points·9 months ago·edited

Small fix — 214nm, not 210. It matters for the comparison you are making with the other run.

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u/joaquin_petrov26 points·9 months ago

Retention time shifted 0.4 minutes and I assumed the worst. It was the column temperature.

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