[Lab] split one vial across PeptideMeter and VendorInvestigate — 93.7% and 94.2%
Right: split one vial across PeptideMeter and VendorInvestigate — 93.7% and 94.2%. I paid for this one myself, nobody sent me anything, and the receipts are in the comments. The numbers the title promised, since a headline without them is worthless: 93.7% and 94.2%. Everything below is context for those. A shoulder…
Left up. The integration disagreement in this thread is the most useful thing on the board this week.
Bought a second-hand instrument and learned more in six months of fixing it than in three years of reading traces.
Correction: that is a tailing factor, not a plate count. Different diagnostics for different problems.
two labs, two gradients, two honest answers
Area percent is the integrated area of your peak over the total integrated area at one wavelength on one gradient. Change any of those and the number changes without the sample changing.
214nm sees the peptide bond, 280nm sees the aromatics
Area percent is the integrated area of your peak over the total integrated area at one wavelength on one gradient.
Adding one practical thing — run the blank. It answers this before anyone has to argue about it.
Retention time shifted 0.4 minutes and I assumed the worst. It was the column temperature.
That is area percent, not mass percent. The trace cannot give you the second one.