[Lab] split one vial across PeptideMeter and VendorInvestigate — 93.7% and 94.2%
Right: split one vial across PeptideMeter and VendorInvestigate — 93.7% and 94.2%. I paid for this one myself, nobody sent me anything, and the receipts are in the comments. The numbers the title promised, since a headline without them is worthless: 93.7% and 94.2%. Everything below is context for those. A shoulder…
System suitability — repeat injections, tailing factor, plate count, RSD on area — is what tells you the instrument was fit for the measurement that day. Without it, the purity figure is unanchored.
a blank injection between samples costs four minutes and settles most arguments
report the method or do not report the number
area percent is relative to what the detector saw and nothing else
area percent is relative to what the detector saw and nothing else
Disagreeing with this bit: that spread is ordinary inter-lab variance, not a disagreement about the material.
Chased a ghost peak for three weeks. It was the plumbing. It is always the plumbing.
Right. And a blank between injections settles the carryover argument before it starts.
Yes. Retention time is a hypothesis about identity. Mass is the answer.