[Question] is 98.6% actually fine or am I being sold a rounding error
Filing this under things worth writing down: is 98.6% actually fine or am I being sold a rounding error. Pulling the figures out of the title: 98.6%. All of it is written down as it happened rather than reconstructed. Mass spectrometry answers identity. UV purity answers relative quantity under the run conditions. A…
Resolution between two peaks depends on retention, selectivity and efficiency. A shallower gradient buys retention and usually resolution, at the cost of peak width and run time.
a shoulder is not an impurity until you can resolve it
Correcting myself upthread: I said the gradient was linear and looking again it has a hold in the middle.
Correcting myself upthread: I said the gradient was linear and looking again it has a hold in the middle.
Adding one practical thing — run the blank. It answers this before anyone has to argue about it.
Started reporting my own integrations with the baseline choice stated. Arguments in my threads dropped by about half.
Same method at both labs, or two different gradients?