[Lab] split one vial across PeptideMeter and VendorInvestigate — 96.8% and 99.1%
Title says it — split one vial across PeptideMeter and VendorInvestigate — 96.8% and 99.1% — so here is the boring supporting detail that makes it worth anything.
The relevant figures are 96.8% and 99.1%, and they come from the same log I have kept the whole time.
A shoulder that does not baseline-resolve cannot be quantified honestly. You can report it as an unresolved shoulder, which is useful information, or you can develop the method until it resolves.
Mass spectrometry answers identity. UV purity answers relative quantity under the run conditions. A document with one and not the other is answering half the question, and the half it answers should be stated.
I will update this if the picture changes rather than quietly leaving it up.
best — the order this archive was captured in
Area percent is the integrated area of your peak over the total integrated area at one wavelength on one gradient. Change any of those and the number changes without the sample changing.
Started reporting my own integrations with the baseline choice stated. Arguments in my threads dropped by about half.
That figure cannot be quoted to two decimals off that baseline. The precision is not in the data.
Cosigning the wavelength point. Half the disagreements in this board are two people looking at different detectors.
Disagree. Inter-lab spread of a point or two on this assay is ordinary and calling it a discrepancy misleads people.
Right. And a blank between injections settles the carryover argument before it starts.
Trace posted without axis labels — asked for a relabelled version rather than removing it.
a blank injection between samples costs four minutes and settles most arguments
LC-MS for identity, UV for relative quantity
LC-MS for identity, UV for relative quantity
insulin_syringe_ian is right — the integration choice is a decision and it should be stated alongside the result.