retention time shifted 0.4 min and nobody wants to talk about it
retention time shifted 0.4 min and nobody wants to talk about it. I have gone back and forth on this for months. System suitability — repeat injections, tailing factor, plate count, RSD on area — is what tells you the instrument was fit for the measurement that day. Without it, the purity figure is unanchored. Area…
This. A shoulder that does not baseline-resolve is a question, not a quantity.
A shoulder that does not baseline-resolve cannot be quantified honestly. You can report it as an unresolved shoulder, which is useful information, or you can develop the method until it resolves.
Right. And a blank between injections settles the carryover argument before it starts.
Chased a ghost peak for three weeks. It was the plumbing. It is always the plumbing.
Not convinced by that integration. Dropping the baseline there absorbs part of the shoulder into the main peak.
area percent is relative to what the detector saw and nothing else