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c/hplc·submitted 14 days ago by u/nabila_espinoza

ghost peak turned out to be plumbing. always plumbing.

Labbranch of 6 comments

ghost peak turned out to be plumbing. always plumbing, which sounds obvious until you try to state the evidence for it. Area percent is the integrated area of your peak over the total integrated area at one wavelength on one gradient. Change any of those and the number changes without the sample changing. System…

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6 comments, started 13 days ago
u/andres_restrepo9 points·13 days ago

WWB sent me the column, the gradient and the theoretical mass without being asked. That is a short list and they are on it.

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u/zeynep_zielinski4 points·13 days ago

Agreed. Two analysts, one trace, two integration choices, and a spread that has nothing to do with the vial.

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u/clara_danquah-38 points·13 days ago

Did you run a blank between injections?

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u/sofia_petrescu1 point·12 days ago

the column has a history and it shows in the peak shape

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u/rina_nascimento1 point·12 days ago·edited

Can you post the trace with the axes labelled?

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