[Lab] LC-MS confirmed identity but the UV purity is 94%. now what
LC-MS confirmed identity but the UV purity is 94%. now what, and before anyone asks: same batch throughout, single submission, no cherry-picking between services. The relevant figures are 94%, and they come from the same log I have kept the whole time. Started reporting my own integrations with the baseline choice…
Resolution between two peaks depends on retention, selectivity and efficiency. A shallower gradient buys retention and usually resolution, at the cost of peak width and run time.
Correcting myself upthread: I said the gradient was linear and looking again it has a hold in the middle.
ghost peak, check the plumbing first, it is always the plumbing
area percent is relative to what the detector saw and nothing else
What did system suitability look like on that sequence?
214nm sees the peptide bond, 280nm sees the aromatics
area percent is relative to what the detector saw and nothing else
ahmed_rasmussen is right — the integration choice is a decision and it should be stated alongside the result.
Right. And a blank between injections settles the carryover argument before it starts.