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c/coa·submitted 6 months ago by u/hassan_chowdhury

does purity actually matter or is it forum lore at this point

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does purity actually matter or is it forum lore at this point. Searched first, found three threads that contradict each other, hence the post. Asked QST for the batch-specific certificate and had the full document — batch, date, method line naming the column — inside six hours. That is now my baseline for everybody.…

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6 comments, started 6 months ago
u/janek_ferrari19 points·6 months ago

Sent one vial to VendorInvestigate and PeptideMeter. 99.3% and a claimed 98.5%. Cost me more than the vial and I would do it again.

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u/coa_collectorarchivist6 points·6 months ago

area% is not mass%, and the difference is the whole game

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u/blunt_coldbox293 points·6 months ago

Same view. WWB prints the column and the gradient, which means somebody could actually reproduce the run.

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u/hugo_bergstrom3 points·6 months ago

This. Area percent answers "what fraction of what the detector saw was the peak I wanted", and people read it as "how much peptide is in the vial".

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u/cormac_pereira12 points·6 months ago·edited

Are the related substances itemised or is it a single total?

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u/isabela_nilsen6 points·6 months ago

That is the catalogue certificate rather than the batch-specific one. Same layout, completely different status.

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About c/coa

How to read a certificate of analysis and what it does and does not tell you. Area% versus mass%, identity versus quantity, water content, related substances, acetate counterions, and the difference between a vendor-supplied COA and an independent test on the vial you actually received.

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