GLPHubglpresearchhub.com
Read-only archive. GLP Research Hub is a static community record — nothing here is for sale, no account is needed, and no vote you cast is counted. Why?
Single comment threadYou are looking at one branch of help me understand purity, I have read the wiki twice — 32 comments in the full submission. View in context.
692
c/coa·submitted 1 months ago by u/tove_ogunleye

help me understand purity, I have read the wiki twice

COAbranch of 10 comments

Asking properly rather than in a comment on somebody else’s thread: help me understand purity, I have read the wiki twice. LC-MS gives you identity; UV purity gives you a relative quantity. A document with the first and not the second, or the reverse, is answering half the question. Asked GL Biochem for the…

Read the full submission and all 32 comments →

This branch

10 comments, started 1 months ago
u/dead_space_doug6 points·1 months ago

Net peptide content is the fraction of the powder that is actually peptide once counterion, water and residual solvent are accounted for. A 99% pure acetate salt can still be about 85% peptide by mass.

replysharereportpermalink
u/tove_ogunleyeOP4 points·1 months ago

the lot on the vial has to match the lot on the page

replysharereportpermalink
[deleted]2 points·1 months ago

[deleted]

replysharereportpermalink
u/amara_halonen1 point·1 months ago

Area percent or net peptide content? They answer different questions.

replysharereportpermalink
u/alcohol_aversion1 point·1 months ago

Started keeping a folder of every certificate I have been sent. The ones that itemise impurities are a small group and they are mostly the same suppliers.

replysharereportpermalink
u/ghrp_history3 points·1 months ago

chromatogram or it did not happen

replysharereportpermalink
u/tove_ogunleyeOP4 points·1 months ago

water content is on there and everyone skips it

replysharereportpermalink
u/ghrp_history1 point·1 months ago

post the whole document, not the headline number

replysharereportpermalink
u/dead_space_doug1 point·1 months ago

Water content 3.8%, acetate 6.2%, purity 99%, net peptide about 90% of label weight. All four numbers honest, and I had to read it twice.

replysharereportpermalink
u/certified_referenceQC1 point·1 months ago

The document I was worried about turned out to be a re-issue with a later creation date. Asked, got the original, entirely ordinary.

replysharereportpermalink

← back to the whole thread

About c/coa

How to read a certificate of analysis and what it does and does not tell you. Area% versus mass%, identity versus quantity, water content, related substances, acetate counterions, and the difference between a vendor-supplied COA and an independent test on the vial you actually received.

71kmembers
130submissions
Aug 2023created
submissions / month, last year
Sponsored

Janoshik Analytical

Independent HPLC and mass spec. The number you get is the number they found.

janoshik.com
Sponsored

GL Biochem (Shanghai)

Custom peptides and amino acids direct from the manufacturer since 1998. ISO 9001 / cGMP. Batch COA every order.

glbiochem.net
c/coa rules
  1. Post the full document. Cropped COAs that hide the batch number or date get removed.
  2. Redact your own name and order id, not the vendor's batch details.
  3. No "is this good?" without saying what the compound and claimed purity are.
  4. Independent community. Nobody here sells anything, and anyone who tries is banned.
  5. Not medical advice. Describe what you did; never prescribe to a stranger.
  6. Claims need evidence. Batch numbers, dated screenshots, independent test reports, or a citation.
  7. No referral links, discount codes or affiliate URLs. Permanent ban, no appeal.
  8. No contact handles, wallet addresses or tracking numbers — they identify people.
  9. Be recognisably decent. Disagree hard, insult nobody.
Moderators
Volunteers. Unpaid, unaffiliated, and reachable through modmail only.
Before you read on

Several compounds discussed on GLP Research Hub are sold for research use only and are not approved for human use anywhere. Nothing here is medical advice and none of it is written by your clinician. If a post reads like an instruction, treat it as a description of what one stranger did.