does water content actually matter or is it forum lore at this point
Trying to get a straight answer on this: does water content actually matter or is it forum lore at this point. LC-MS gives you identity; UV purity gives you a relative quantity. A document with the first and not the second, or the reverse, is answering half the question. UV at 214nm sees the peptide bond and picks up…
Net peptide content is the fraction of the powder that is actually peptide once counterion, water and residual solvent are accounted for. A 99% pure acetate salt can still be about 85% peptide by mass.
Left up and flaired Explainer. This is the answer we keep linking, written properly.
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What does the method line actually say? Not the technique — the column and the gradient.
Cosigning. The batch identifier is load-bearing, and a certificate that cannot be tied to the vial in your hand is not about your vial.