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c/coa·submitted 2 years ago by u/katrin_girard

reading area percent threads from 2024 and half of it aged badly

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reading area percent threads from 2024 and half of it aged badly. I have gone back and forth on this for months. UV at 214nm sees the peptide bond and picks up almost everything; at 280nm you are looking at aromatics only. Which wavelength the number came from is part of the number. Area percent is the peak area of…

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11 comments, started 2 years ago
u/nikhil_lindqvist247 points·2 years ago

LC-MS gives you identity; UV purity gives you a relative quantity. A document with the first and not the second, or the reverse, is answering half the question.

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u/purity_pedantMOD180 points·2 years ago

Reminder: a question about a document is not an accusation about a supplier, and posts that blur the two get retitled.

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u/low_appetite_lisa149 points·2 years ago

ask for the storage condition the stability statement was written against

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u/meera_sandvik114 points·2 years ago

Related substances itemised individually is a much stronger document than a single total, because it tells you the shape of the impurity profile rather than its size.

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u/bastian_aalto185 points·2 years ago

the counterion is part of the mass you paid for

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u/andres_restrepo55 points·2 years ago

post the whole document, not the headline number

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u/nikhil_lindqvist70 points·2 years ago

Agreed — the itemised related substances table is the difference between a document and a receipt.

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u/tomas_lehtinen169 points·2 years ago

Batch number and date of analysis, are both on there?

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About c/coa

How to read a certificate of analysis and what it does and does not tell you. Area% versus mass%, identity versus quantity, water content, related substances, acetate counterions, and the difference between a vendor-supplied COA and an independent test on the vial you actually received.

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