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c/coa·submitted 2 years ago by u/emil_agyeman

how much of what we believe about COA actually comes from area percent threads

Suspiciousbranch of 12 comments

Genuine question, and the title is the question: how much of what we believe about COA actually comes from area percent threads. My first certificate had a compound name, a percentage and a logo. I thought that was normal. It is not, and now I know what to ask for. Asked for the storage condition behind the stability…

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12 comments, started 2 years ago
u/cormac_pereira123 points·2 years ago

This. Area percent answers "what fraction of what the detector saw was the peak I wanted", and people read it as "how much peptide is in the vial".

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u/first_hundredMOD71 points·2 years ago

Removed — cropped document. Post the whole page or do not post it; the fields people crop are the fields that matter.

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u/noor_cardoso101 points·2 years ago·edited

Small fix — 214nm, not 280nm. It matters here because the impurity you are asking about barely absorbs at the longer wavelength.

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u/wanjiru_varga84 points·2 years ago

Correction: that is net peptide content, not purity. Different number, different meaning, and the document says so if you read the row label.

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u/dario_boateng92 points·2 years ago

The PDF creation date being later than the analysis date is usually a re-issue or a re-export. It is worth one question and it is not evidence of anything on its own.

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[removed]37 points·2 years ago

[removed by moderator]

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u/emil_agyemanOP14 points·2 years ago

Does the lot on the vial match the lot on the page?

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u/emil_agyemanOP5 points·2 years ago

Correcting myself upthread: I said the date was missing and it is on the second page, which I had not scrolled to.

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u/hugo_bergstrom4 points·2 years ago

Careful — "the metadata looks odd" is a reason to ask a question, not a conclusion. Re-issues are ordinary.

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u/emeka_chowdhury47 points·2 years ago

identity and quantity are two questions and most documents answer one

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u/cesar_ivaturi29 points·2 years ago

That is the catalogue certificate rather than the batch-specific one. Same layout, completely different status.

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u/liv_nkemelu16 points·2 years ago

Right, and net peptide content is where the counterion and the water go. Both numbers can be honest and very different.

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About c/coa

How to read a certificate of analysis and what it does and does not tell you. Area% versus mass%, identity versus quantity, water content, related substances, acetate counterions, and the difference between a vendor-supplied COA and an independent test on the vial you actually received.

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