acetate counterion is why your 10mg vial has 8.7mg of peptide
Posting this as a discussion rather than a claim: acetate counterion is why your 10mg vial has 8.7mg of peptide.
Numbers, in the order they matter: 10mg and 8.7mg.
UV at 214nm sees the peptide bond and picks up almost everything; at 280nm you are looking at aromatics only. Which wavelength the number came from is part of the number.
Related substances itemised individually is a much stronger document than a single total, because it tells you the shape of the impurity profile rather than its size.
Screenshot none of this. Read the whole thread, including the parts where I am told I am wrong.
best — the order this archive was captured in
Area percent is the peak area of your compound over the total integrated area, at one wavelength, on one gradient. It is a relative measure of what the detector saw, not a mass fraction of the vial.
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Are the related substances itemised or is it a single total?
area% is not mass%, and the difference is the whole game
Is water content on the document? It changes your concentration arithmetic.
LC-MS gives you identity; UV purity gives you a relative quantity. A document with the first and not the second, or the reverse, is answering half the question.
The PDF creation date being later than the analysis date is usually a re-issue or a re-export. It is worth one question and it is not evidence of anything on its own.
- 1Area percent is the peak area of your compound over the total integrated…7 comments in this branch · started by u/yusuf_nakamura