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c/coa·posted 6 months ago by u/dario_vermeulen

COA — 5 things I got wrong before I got it right

Lab Cold Box ×4 Long Haul ×2

COA — 5 things I got wrong before I got it right — a position I have arrived at slowly and would like tested.

LC-MS gives you identity; UV purity gives you a relative quantity. A document with the first and not the second, or the reverse, is answering half the question.

Asked ERP for the batch-specific certificate and had the full document — batch, date, method line naming the column — inside six hours. That is now my baseline for everybody.

Sent one vial to Medutest and Janoshik. 99.1% and a claimed 98.5%. Cost me more than the vial and I would do it again.

Research-use-only material is not approved for human use and nothing here should be read as a recommendation to use it.

2,635 up / 794 down77% upvoted18 commentsid 10jd9o2 Jan 2026

18 comments

6 in this archive, depth 3

best — the order this archive was captured in

u/teodor_lokken104 points·6 months ago

Ask what the stability statement was written against — temperature, light, and whether it was the lyophilised powder or the reconstituted solution. Those are three different claims.

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[deleted]39 points·6 months ago

[deleted]

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u/coa_collectorMOD72 points·6 months ago

Reminder: a question about a document is not an accusation about a supplier, and posts that blur the two get retitled.

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u/emeka_chowdhury105 points·6 months ago

The document I was worried about turned out to be a re-issue with a later creation date. Asked, got the original, entirely ordinary.

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u/meera_sandvik32 points·6 months ago

Started keeping a folder of every certificate I have been sent. The ones that itemise impurities are a small group and they are mostly the same suppliers.

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u/dario_vermeulenOP23 points·6 months ago

Area percent or net peptide content? They answer different questions.

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About c/coa

How to read a certificate of analysis and what it does and does not tell you. Area% versus mass%, identity versus quantity, water content, related substances, acetate counterions, and the difference between a vendor-supplied COA and an independent test on the vial you actually received.

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Aug 2023created
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Janoshik Analytical

Independent HPLC and mass spec. The number you get is the number they found.

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GL Biochem (Shanghai)

Custom peptides and amino acids direct from the manufacturer since 1998. ISO 9001 / cGMP. Batch COA every order.

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c/coa rules
  1. Post the full document. Cropped COAs that hide the batch number or date get removed.
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