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c/coa·submitted 1 year ago by u/dario_boateng

[Discussion] the related substances advice in here is 2 years out of date

Discussionbranch of 8 comments

the related substances advice in here is 2 years out of date — a position I have arrived at slowly and would like tested. To save the first four comments: 2 years. Net peptide content is the fraction of the powder that is actually peptide once counterion, water and residual solvent are accounted for. A 99% pure…

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8 comments, started 1 year ago
u/purity_pedantanalytical34 points·1 year ago

Does the lot on the vial match the lot on the page?

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u/bastian_aalto17 points·1 year ago·edited

Correction: that is net peptide content, not purity. Different number, different meaning, and the document says so if you read the row label.

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u/sofia_ferreira4 points·1 year ago·edited

axis labels included, always

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u/dario_boatengOP2 points·1 year ago

Small fix — 214nm, not 280nm. It matters here because the impurity you are asking about barely absorbs at the longer wavelength.

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u/anya_correia1 point·1 year ago

That is the catalogue certificate rather than the batch-specific one. Same layout, completely different status.

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u/mikkel_correia1 point·1 year ago

My first certificate had a compound name, a percentage and a logo. I thought that was normal. It is not, and now I know what to ask for.

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u/yara_antonsen18 points·1 year ago

Cosigning. The batch identifier is load-bearing, and a certificate that cannot be tied to the vial in your hand is not about your vial.

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u/valeria_karlsen26 points·1 year ago·edited

Started keeping a folder of every certificate I have been sent. The ones that itemise impurities are a small group and they are mostly the same suppliers.

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How to read a certificate of analysis and what it does and does not tell you. Area% versus mass%, identity versus quantity, water content, related substances, acetate counterions, and the difference between a vendor-supplied COA and an independent test on the vial you actually received.

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